NRAS - zest. do oceny mutacji


 

NRAS Mutations and Cancer


NRAS is a member of the RAS family of GTPases and plays a central role in the MAPK signaling pathway. Activating mutations in codons 12, 13, and 61 have been found in various cancers, including melanoma (13-25%), colorectal cancer (1-6%), lung cancer (1%), hepatocellular carcinoma (10%), myeloid leukemias (14%), and thyroid carcinoma (7%)

 

The table below lists the mutations detected by this kit.

 

Exon

Mutation Name

Nucleotide Change

Amino Acid Change

2

G12C

c.34G>T

Glycine (G) to Cystein (C)

G12D

c.35G>A

Glycine (G) to Aspartic acid (D)

G12S

c.34G>A

Glycine (G) to Serine (S)

G13V

c.38G>T

Glycine (G) to Valine (V)

G13R

c.37G>C

Glycine (G) to Arginine (R)

3

Q61K

c.181C>A

Glutamine (Q) to Lysine (K)

Q61R

c.182A>G

Glutamine (Q) to Arginine (R)

Q61L

c.182A>T

Glutamine (Q) to Leucine (L)

Q61H

c.183A>C & c.183A>T

Glutamine (Q) to Histidine (H)

4

A146T

c.436G>A

Alanine (A) to Threonine (T)

 

 

EntroGen’s NRAS mutation detection kit is a polymerase chain reaction (PCR)-based assay that uses allele-specific primers to identify the presence of the mutations listed above. The testing procedure involves three (3) simple steps:

  • Isolation of DNA from tumor biopsies, paraffin-embedded sections (FFPE), or fresh frozen tumors
  • Amplification of regions of the NRAS gene using allele-specific primers.
  • Detection of amplification product on a Real-Time PCR instrument.

This test can be completed in approximately 2 hours from isolation of DNA to test result.

 

Equipment and Materials
EntroGen’s NRAS mutation detection kit requires a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes.

This test includes reagents required for PCR amplification/detection, as well as validated reaction controls. Columns and reagents for DNA isolation are not included.

 

Intended Use
EntroGen’s NRAS mutation detection kit is for Research Use Only (RUO) and CE-IVD.

 

Certyfikacja CE-IVD.

 

Więcej informacji na stronie EntroGen, Inc. USA