EGFR - zest. do oceny mutacji


EGFR Mutation Analysis Kit

Available Products

 

EGFR Mutations and Cancer

Epidermal growth factor receptor (EGFR) is a membrane protein that plays a central role in transmitting signals that promote cell growth and proliferation. Its tyrosine kinase (TK) domain activates several downstream effectors that lead to activation of the Ras-Raf-MAPK pathway. Overexpression and oncogenic mutations that constitutively activate the TK domain of EGFR have been found in various solid tumors. Additionally, excessive activation of EGFR has been shown to be associated with advanced stages of cancer and a poor prognosis.

Testing Procedure and Analysis

EntroGen's EGFR mutation analysis kit is a real-time polymerase chain reaction (PCR)-based assay that uses mutant-specific probes to identify the presence of EGFR mutations. The testing procedure involves three (3) simple steps, which can be completed in approximately 2 hours from DNA to test results:

Isolation of DNA from tumor biopsies, paraffin-embedded sections (FFPE), fresh frozen tumors, or tumor cell lines.PCR setup with reagents included in the kit.Amplification (8 reactions per sample) and detection using a real-time PCR instrument.

Specifications

EntroGen's EGFR mutation analysis kit detects the following EGFR mutations:

 

Exon 18

  • G719A - 2156G>C
  • G719S - 2155G>A
  • G719C - 2155G>T

Exon 19

  • 2235-2249 del 15
  • 2235-2252>AAT del18
  • 2236-2253 del 18
  • 2237-2251 del 15
  • 2237-2254 del 18
  • 2237-2255>T del 19
  • 2236-2250 del 15
  • 2238-2255 del 18
  • 2238-2248>GC del 11
  • 2238-2252>GCA del 15
  • 2239-2247 del 9
  • 2239-2253 del 15
  • 2239-2256 del 18
  • 2239-2248>C del 10
  • 2239-2258>CA del 20
  • 2240-2251 del 12
  • 2240-2257 del 18
  • 2240-2254 del 15
  • 2239-2251>C del 13

Exon 20

  • T790M - 2369C>T
  • S768I - 2303G>T
  • 2307-2308 ins GCCAGCGTG
  • 2319-2320 ins CAC
  • 2310-2311 ins GGT

Exon 21

  • L858R - 2573T>G
  • L861Q - 2582T>A

 

Limitations

EntroGen's EGFR mutation analysis kit requires 40-80 nanograms of genomic DNA (5-10 nanograms per reaction) and can detect a mutation load of less than 1%. This sensitivity greatly depends on the extent of fragmentation and quality of the isolated DNA.

 

Equipment and Materials

EntroGen's EGFR mutation analysis kit requires a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes.

This test includes reagents required for the PCR amplification/detection, as well as validated reaction controls. Columns and reagents for DNA isolation are not included.

 

Intended Use

EntroGen's EGFR mutation analysis kit is available for research (RUO) and diagnostic (CE-IVD) purposes.

 

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